Catalysis of the reaction: tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.
Source:PMID:2455297,
RHEA:10628
Comment
Note that, although EC names the enzme tRNA nucleotidyltransferase, the enzyme to which this term refers is also known as 'ribonuclease PH', and degrades tRNA in the 3'-5' direction in vivo, the term does not have parentage in the 'nuclease activity' branch of the ontology because both GO and the Enzyme Commission define nuclease activity as a type of hydrolysis It should not be confused with CCA tRNA nucleotidyltransferase..