1. Extract storage proteins (glutenins/gliadins) from wheat grain; 2. Separate glutenins from gliadins; 3. Run proteins extracts in polyacrylamide gels; 4. Stain the gel and de-stain; 5. Identify the allele for each locus.
Source:
CO:curators
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CO_321:0000390 wheat sds-page electrophoresis method