The chlorophyll content of leaves (and other green tissues) can be quickly, and non-destructively measured using a hand-held battery portable optical meter (e.g., Minolta SPAD-502 chlorophyll meter) which measures the chlorophyll content via light transmittance (absorbance of red light at 650 nm and infrared light at 940 nm) and compensates for differing leaf thicknesses. Readings from the instrument are not absolute chlorophyll values, instead each reading is a ‘chlorophyll concentration index’ (CCI; ranging from 0 to 99.9). Measurements are typically made on the flag leaf (once fully expanded), although measurements of lower leaves may be taken to assess canopy chlorophyll profiles.
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CO_321:0000249 wheat chl measurement method