Place non-dormant grains on moistened filter paper and cover with a petri-dish lid during germination. After the coleoptiles have reached a length of about 5 mm in darkness, place them in artificial light (daylight equivalent) at 750-1250 lux continuously for 3 to 4 days, at a temperature of 25 to 30 degrees Centigrade. Observe colour when the coleoptile is fully with first leaf just emerging (about 6 to 7 days)
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CO_320:0000548 rice coleoptile anthocyanin evaluation method