Step 1 (pollen fertility: see pollen sterility score): Collect pollen from at least ten florets at anthesis, fix in 70% alcohol. Place 2-3 anthers from each of five florets on a glass slide, and squeeze the pollen out in a drop of 1% iodine potassium iodide (I2-KI) solution with a spear-shaped needle. Using a microscope at magnification 10x10, count fertile and sterile grains in at least three microscopic fields, calculate the percentage that are fertile, and score. Step 2 (spikelet fertility): count the number of filled grains andtotal spikelets per panicle and convert into percentage fertile. Score the combination of pollen fertility with spikelet fertility
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