Collect pollen from at least ten florets at anthesis, fix in 70% alcohol. Place 2-3 anthers from each of five florets on a glass slide, and squeeze the pollen out in a drop of 1% iodine potassium iodide (I2-KI) solution with a spear-shaped needle. Fertile pollen grains will be round and well stained, sterile pollen grains will be unstained or partially stained, and withered or round. Using a microscope at magnification 10x10, count fertile and sterile grains in at least three microscopic fields, calculate the proportion that are sterile
[source: unknown]
Comment
None
Subset
None
Related
Link
to all annotated objects annotated to rice pollen sterility calculation method.
Link
to all direct and indirect annotations
to rice pollen sterility calculation method.
Link
to all direct and indirect annotations download (limited to first 10,000)
for rice pollen sterility calculation method.