Place 0.1g ground rice in a test tube with thymol blue (0.025% in ethanol, 0.2 ml) and KOH (0.2N, 2 ml). Shake the tube to mix contents, boil (8 min), rest (5 min) and then place in an ice-bath (15 min). Lay cooled tubes flat on graph paper for 1 hour, and then measure the distance that the gel travels . Included standards, as the age of the rice, degree of milling and operator skill affect reproducibility: IR48 (85?90 mm), IR32 (72-75 mm) and IR42 (30-32 mm).
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