Sampling is typically performed at sequential developmental stages/time intervals through crop growth. Cut all aboveground biomass in a predetermined area (A). Avoid border effects by sampling away from edges of plot. In most cases, determinations of dry mass are made on representative sub-samples to reduce oven space requirement, take additional measurements (e.g., fertile culm count) etc. Several protocols available (Bell and Fischer, 1994; Reynolds et al., 2001; Pask et al., 2012), decide which method suit better for your objectives.
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CO_321:0000394 wheat isbm measurement method