Proceed following the protocol for sampling of grains or leaves for CID in Chapter 6 in Pask et al., 2012. Mass spectrometers generate differential values of stable carbon isotope composition (δ13C), expressed negative values in parts per thousand (‰) (Farquhar et al., 1989): δ13C (‰) = [(Rsample/Rstandard)–1] × 1000. Where: the ratio of heavy to light isotope (R = 13C/12C) for the sample is in comparison to Peedee Belemnite (PDB) carbonate (limestone) standard. Following Farquhar et al. (1989) – rather than using carbon isotope composition values (δ13C), positive values for CID (Δ13C) can be calculated for easier statistical analysis as: Δ13C = [(δa-δp)]/[1 + (δp/1000)]
Source:
CO:curators
Comment
None
Subset
None
Related
Link
to all annotated objects annotated to wheat cid determination method.
Link
to all direct and indirect annotations
to wheat cid determination method.
Link
to all direct and indirect annotations download (limited to first 10,000)
for wheat cid determination method.
![[Current term]](https://browser.planteome.org/static/images/term_lineage_wedge.png "Current term")
CO_321:0000383 wheat cid determination method